role of ph on adhesion of trichomonas vaginalis isolated from symptomatic & asymptomatic women to vaginal epithelial cells in vitro
نویسندگان
چکیده
background : vaginal epithelium is the most important site for initial contact of the parasite infection in humans. objective: to investigate the effect of lactobacillus acidophilus (la) and ph on attachment of t. vaginalis isolated from symptomatic and asymptomatic patients to vaginal epithelial cells (vecs). methods: following 1-4 hrs contact of parasite with vecs, wet mounts were prepared and used for measuring cell adherence in vitro. this was done by addition of whole la, its excretory secretory product (esp) and changing the ph of the media either by hcl or la. the mean of the three readings of the experiments was taken to compare the isolates and their controls. results: the exposure of vecs to whole la for one hour led to enhancement of adhesion and then a gradual fall with ultimately non-viability t.vaginalis after 4 hrs of incubation with t. vaginalis . in the presence of esp a gradual decreased of adhesion was observed. the number of vecs attached by t.vaginalis was higher in symptomatic than asymptomatic women’s isolates when ph was reduced to 4.5 by hcl, when ph was reduced to a similar level by la this ratio was 47% and 35% respectively. conclusion: the presence of la enhanced the attachment of parasite at initial steps of infection, but its effect was deleterious thereafter. this study should be paralleled by appropriate in vivo experiments and if substantiated can be used for effective therapy and prevention of human trichomoniasis.
منابع مشابه
Role of pH on Adhesion of Trichomonas Vaginalis Isolated from Symptomatic & Asymptomatic Women to Vaginal Epithelial Cells in Vitro
Background: Vaginal epithelium is the most important site for initial contact of the parasite infection in humans. Objective: To investigate the effect of Lactobacillus acidophilus (LA) and pH on attachment of T. vaginalis isolated from symptomatic and asymptomatic patients to vaginal epithelial cells (VECs). Methods: Following 1-4 hrs contact of parasite with VECs, wet mounts were prepared and...
متن کاملRole of pH on Adhesion of Trichomonas Vaginalis Isolated from Symptomatic & Asymptomatic Women to Vaginal Epithelial Cells in Vitro
Background: Vaginal epithelium is the most important site for initial contact of the parasite infection in humans. Objective: To investigate the effect of Lactobacillus acidophilus (LA) and pH on attachment of T. vaginalis isolated from symptomatic and asymptomatic patients to vaginal epithelial
متن کاملthe role of russia in transmission of energy from central asia and caucuses to european union
پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...
15 صفحه اولMorphological studies of the cytotoxicity of Trichomonas vaginalis to normal human vaginal epithelial cells in vitro.
In vitro cultured monolayers of normal human vaginal epithelial cells were incubated with axenic cultures of Trichomonas vaginalis. Two strains freshly isolated from patients with trichomoniasis and one strain that had been maintained in axenic culture for several years were studied. Freshly isolated trichomonads showed amoeboid movements, adherence to epithelial cell surfaces, and were cytotox...
متن کاملCytopathogenic effect of Trichomonas vaginalis on human vaginal epithelial cells cultured in vitro.
In this study we established human vaginal epithelial cells (hVECs) in culture and evaluated their interaction with Trichomonas vaginalis parasites to complement previous studies using other cell types. Primary cultures of hVECs were established. Contaminating fibroblasts were separated from epithelial cells by differential trypsinization. Specific antibody staining revealed that over 92% of ce...
متن کامل"evaluation of trichomonas vaginalis isolates from symptomatic and asymptomatic patients in mouse model"
trichomoniasis, a worldwide prevalent infection, is a perfect example of interplay between the infecting parasite and the host, on which the presentation of disease depends. to study the pathogenesis, animal model is required for establishment of infection. in this study, by using strains of trichomonas vaginalis isolated from vaginal swabs and/or urine samples and maintained in axenic form fro...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of medical sciencesجلد ۲۹، شماره ۳، صفحات ۱۳۴-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023